Extracting RNA and DNA from Mdec - ENCORE Priming - 052325

Extracting RNA and DNA from fragments of Madracis decactis for the ENCORE priming project of Florence

Protocols used

I’m extracting RNA and DNA from frozen fragments of using the Zymo Quick-DNA/RNA Miniprep Plus Kit and the protocol linked above. The kit protocol is found here.

  • I eluted RNA samples in 80ul and DNA samples in 80ul.

Qubit Results I used Broad range RNA Qubit Protocol linked above. DNA samples will be measured after finishing with all extractions (for all samples). RNA samples were read twice, standard only read once.

  • Standard 1: 482.51
  • Standard 2: 10262.94
Extraction date   colony_id   Species   RNA_read1   RNA_read2   RNA_AVG (ng/ul)
20250523   MD-2-4   Madracis decactis   10.2   10.1   10.5
20250523   MD-1-15   Madracis decactis   15.4   14.9   15.15
20250523   MD-1-5   Madracis decactis   14.4   14.4   14.4
20250523   MD-4-6   Madracis decactis   10.2   9.8   10.0

Gel

  • I used the protocol linked above with few modifications. I ran 11ul of RNA and 9ul of DNA. I added 4ul of gel green to the gel.

Note: I might need to use more than 11ul RNA as the samples are difficult to see.

Gel_DNA_RNA_23May2025.png

Written on May 23, 2025