Extracting RNA and DNA from Mdec - ENCORE Priming - 052625

Extracting RNA and DNA from fragments of Madracis decactis for the ENCORE priming project of Florence - 052625

Protocols used

I’m extracting RNA and DNA from frozen fragments of using the Zymo Quick-DNA/RNA Miniprep Plus Kit and the protocol linked above. The kit protocol is found here.

  • I eluted RNA samples in 80ul and DNA samples in 80ul.

Qubit Results I used Broad range RNA Qubit Protocol linked above. DNA samples will be measured after finishing with all extractions (for all samples). RNA samples were read twice, standard only read once.

  • Standard 1: 437.26
  • Standard 2: 7967.46
Extraction date   colony_id   Species   RNA_read1   RNA_read2   RNA_AVG (ng/ul)
20250526   MD-4-13   Madracis decactis   16.6   16.5   16.55
20250526   MD-4-1   Madracis decactis   25.6   24.2   24.9
20250526   MD-2-11   Madracis decactis   23.0   23.0   23.0
20250526   MD-2-5   Madracis decactis   18.8   16.4   17.6
20250526   MD-2   Madracis decactis   19.4   19.4   19.4
20250526   MD-1   Madracis decactis   20.8   20.6   20.7

Gel

  • I used the protocol linked above with few modifications. I ran 12ul of RNA and 9ul of DNA. I added 4ul of gel green to the gel.

Gel_DNA_RNA_26May2025_FS.png

Written on May 26, 2025