Extracting RNA and DNA from Mdec - ENCORE Priming - 052725

Extracting RNA and DNA from fragments of Madracis decactis for the ENCORE priming project of Florence - 052725

Protocols used

I’m extracting RNA and DNA from frozen fragments of using the Zymo Quick-DNA/RNA Miniprep Plus Kit and the protocol linked above. The kit protocol is found here.

  • I eluted RNA samples in 80ul and DNA samples in 80ul.

Qubit Results I used Broad range RNA Qubit Protocol linked above. DNA samples will be measured after finishing with all extractions (for all samples). RNA samples were read twice, standard only read once.

  • Standard 1: 480.39
  • Standard 2: 10061.03
Extraction date   colony_id   Species   RNA_read1   RNA_read2   RNA_AVG (ng/ul)
20250527   MD-1-3   Madracis decactis   17.6   15.2   16.4
20250527   MD-5-5   Madracis decactis   11.2   10.8   11.0
20250527   MD-2-23   Madracis decactis   LOW   LOW   LOW
20250527   MD-1-11   Madracis decactis   LOW   LOW   LOW
20250527   MD-1-20   Madracis decactis   10.6   10.1   10.35
20250527   MD-4-9   Madracis decactis   16.0   15.8   15.9

Gel

  • I used the protocol linked above with few modifications. I ran 12ul of RNA and 9ul of DNA. I added 4ul of gel green to the gel. Note: I need to re-extract MD-2-23 and MD-1-11

Gel_DNA_RNA_27May2025_FS.jpg

Written on May 27, 2025