Extracting RNA and DNA from Mdec - ENCORE Priming - 052925

Extracting RNA and DNA from fragments of Madracis decactis for the ENCORE priming project of Florence - 052925

Protocols used

I’m extracting RNA and DNA from frozen fragments of using the Zymo Quick-DNA/RNA Miniprep Plus Kit and the protocol linked above. The kit protocol is found here.

  • I eluted RNA samples in 80ul and DNA samples in 80ul.

Qubit Results I used Broad range RNA Qubit Protocol linked above. DNA samples will be measured after finishing with all extractions (for all samples). RNA samples were read twice, standard only read once.

  • Standard 1: 398.44
  • Standard 2: 9037.49
Extraction date   colony_id   Species   RNA_read1   RNA_read2   RNA_AVG (ng/ul)
20250527   MD-2-23   Madracis decactis   10.2   10.1   10.15
20250527   MD-1-11   Madracis decactis   12.3   12.4   12.35
20250527   MD-2-6   Madracis decactis   16.1   16.0   16.05
20250527   MD-5-21   Madracis decactis   17.5   17.0   17.25
20250527   MD-4-2   Madracis decactis   10.4   10.2   10.3
20250527   MD-4-26   Madracis decactis   11.5   11.3   11.4

Gel

  • I used the protocol linked above with few modifications. I ran 12ul of RNA and 9ul of DNA. I added 4ul of gel green to the gel. Note: In the picture took with the phone the first sample looks super faint but it is there. The last two samples where extracted from Natalie (1-24 and 9-5)

Gel_DNA_RNA_29May2025_FS.jpg

Written on May 29, 2025