Extracting RNA and DNA from Mdec - ENCORE Priming - 060225

Extracting RNA and DNA from fragments of Madracis decactis for the ENCORE priming project of Florence - 060225

Protocols used

I’m extracting RNA and DNA from frozen fragments of using the Zymo Quick-DNA/RNA Miniprep Plus Kit and the protocol linked above. The kit protocol is found here.

  • I eluted RNA samples in 80ul and DNA samples in 80ul.

Qubit Results

I used Broad range RNA Qubit Protocol linked above. DNA samples will be measured after finishing with all extractions (for all samples). RNA samples were read twice, standard only read once.

  • Standard 1: 461.65
  • Standard 2: 9839.73
Extraction date   colony_id   Species   RNA_read1   RNA_read2   RNA_AVG (ng/ul)
20250602   MD-5-19   Madracis decactis   12.0   12.0   12.0
20250602   MD-1-25   Madracis decactis   10.8   10.8   10.8
20250602   MD-2-24   Madracis decactis   11.2   11.2   11.2
20250602   MD-4-17   Madracis decactis   11.2   11.0   11.1

Gel

  • I used the protocol linked above with few modifications. I ran 12ul of RNA and 9ul of DNA. I added 4ul of gel green to the gel.

Gel_DNA_RNA_02June2025_FS.jpg

Written on June 2, 2025