Continuing extracting RNA and DNA from Pacu, Mcap, Pcomp - Hawai TPC - 121624

Extracting RNA and DNA from fragments of Pocillopora acuta, Montipora capitata, Porites compressa for the Hawaii TPC project

Protocols used

I’m continuing extracting RNA and DNA from frozen fragments of Pacuta, Mcap and Pcomp using the Zymo Quick-DNA/RNA Miniprep Plus Kit and the protocol linked above. The kit protocol is found here.

  • I eluted RNA and DNA samples in 80ul.
  • I re-extracted here sample Mcap G6, in the first extraction there seemed to be DNA in the RNA sample (from the gel)

Qubit Results

I used Broad range RNA Qubit Protocol inked above. I will check the DNA samples after finishing with all extractions (for all samples). RNA samples were read twice, standard only read once.

  • Standard 1: 485.09
  • Standard 2: 10423.38
Clipping date   Extraction date   colony_id   Species   Temp.Cat   RNA_read1   RNA_read2   RNA_AVG (ng/ul)
20241203   20241216   Pcom-H7   Porites compressa   35   31.8   30.4   31.1
20241203   20241216   Mcap-F7   Montipora capitata   35   21.6   20.8   21.2
20241202   20241216   Pacu-F10   Pocillopora acuta   26.8   14.4   14.4   14.4
20241129   20241216   Pacu-B6   Pocillopora acuta   12   23.2   22.2   22.7
  • Standard 1: 459.78
  • Standard 2: 10345.31
Clipping date   Extraction date   colony_id   Species   Temp.Cat   RNA_read1   RNA_read2   RNA_AVG (ng/ul)
20241129   20241216   Mcap-G6   Montipora capitata   12   26.8   25.4   26.1
20241203   20241216   Pcom-B8   Porites compressa   25   43.6   40.4   42.0
20241203   20241216   Pcom-H8   Porites compressa   25   53.2   54.4   53.8
20241203   20241216   Pacu-B9   Pocillopora acuta   30   40.2   37.0   38.6

Gel

  • I used the protocol linked above with few modifications. I ran 11ul of RNA and 9ul of DNA. I added 4ul of gel green to the gel.

Gel_DNA_RNA_16Dec2024.png

Written on December 16, 2024