Continuing extracting RNA and DNA from Pacu, Mcap, Pcomp - Hawai TPC - 120524

Extracting RNA and DNA from fragments of Pocillopora acuta, Montipora capitata, Porites compressa for the Hawaii TPC project

Protocols used

I’m continuing extracting RNA and DNA from frozen fragments of Pacuta, Mcap and Pcomp using the Zymo Quick-DNA/RNA Miniprep Plus Kit and the protocol linked above. The kit protocol is found here.

  • I eluted RNA and DNA samples in 80ul.

Qubit Results I used Broad range RNA Qubit Protocol inked above. I will check the DNA samples after finishing with all extractions (for all samples). RNA samples were read twice, standard only read once.

  • Standard 1: 432.51
  • Standard 2: 10683.02
Clipping date   Extraction date   colony_id   Species   Temp.Cat   RNA_read1   RNA_read2   RNA_AVG (ng/ul)
20241202   20241205   Pacu-H8   Pocillopora acuta   25   27.4   26.8   27.1
20241202   20241205   Pacu-G1   Pocillopora acuta   18   61.0   59.6   60.3
20241203   20241205   Mcap-H9   Montipora capitata   30   28.4   26.8   27.6
20241129   20241205   Pcom-H6   Porites compressa   12   16.4   16.0   16.2
  • Standard 1: 496.25
  • Standard 2: 10161.14
Clipping date   Extraction date   colony_id   Species   Temp.Cat   RNA_read1   RNA_read2   RNA_AVG (ng/ul)
20241129   20241205   Pcom-B6   Porites compressa   12   23.0   19.4   21.2
20241203   20241205   Mcap-B9   Montipora capitata   30   19.0   18.4   18.7
20241203   20241205   Mcap-G7   Montipora capitata   35   18.6   17.0   17.8
20241202   20241205   Pacu-G10   Pocillopora acuta   26.8   30.0   29.8   29.9

Gel

  • I used the protocol linked above with few modifications. I ran 11ul of RNA and 9ul of DNA. I added 4ul of gel green to the gel.

Gel_DNA_RNA_05Dec2024.png

Written on December 5, 2024