Continuing extracting RNA and DNA from Pacu, Mcap, Pcomp - Hawai TPC - 120924

Extracting RNA and DNA from fragments of Pocillopora acuta, Montipora capitata, Porites compressa for the Hawaii TPC project

Protocols used

I’m continuing extracting RNA and DNA from frozen fragments of Pacuta, Mcap and Pcomp using the Zymo Quick-DNA/RNA Miniprep Plus Kit and the protocol linked above. The kit protocol is found here.

  • I eluted RNA and DNA samples in 80ul.
  • Note, the DNA samples came out in a much bigger volume (must have added more volume by mistake, the gel looks ok though)

Qubit Results I used Broad range RNA Qubit Protocol inked above. I will check the DNA samples after finishing with all extractions (for all samples). RNA samples were read twice, standard only read once.

  • Standard 1: 432.49
  • Standard 2: 8043.39
Clipping date   Extraction date   colony_id   Species   Temp.Cat   RNA_read1   RNA_read2   RNA_AVG (ng/ul)
20241129   20241209   Pacu-H6   Pocillopora acuta   12   LOW   LOW    
20241202   20241209   Mcap-B1   Montipora capitata   18   18.8   18.4   18.6
20241203   20241209   Mcap-E9   Montipora capitata   30   22.8   22.4   22.6
20241203   20241209   Pcom-E8   Porites compressa   25   49.0   47.2   48.1
  • Standard 1: 464.66
  • Standard 2: 8636.78
Clipping date   Extraction date   colony_id   Species   Temp.Cat   RNA_read1   RNA_read2   RNA_AVG (ng/ul)
20241203   20241209   Mcap-F8   Montipora capitata   25   29.4   28.8   29.1
20241203   20241209   Pcom-G9   Porites compressa   30   64.0   61.8   62.9
20241202   20241209   Pcom-F10   Porites compressa   26.8   44.0   44.0   44.0
20241202   20241209   Pacu-E8   Pocillopora acuta   25   26.0   25.6   25.8
  • Note: will re-extract Pacu-H6

Gel

  • I used the protocol linked above with few modifications. I ran 11ul of RNA and 9ul of DNA. I added 4ul of gel green to the gel.

Gel_DNA_RNA_09Dec2024.png

Written on December 9, 2024