Continuing extracting RNA and DNA from Pacu, Mcap, Pcomp - Hawai TPC - 121024

Extracting RNA and DNA from fragments of Pocillopora acuta, Montipora capitata, Porites compressa for the Hawaii TPC project

Protocols used

I’m continuing extracting RNA and DNA from frozen fragments of Pacuta, Mcap and Pcomp using the Zymo Quick-DNA/RNA Miniprep Plus Kit and the protocol linked above. The kit protocol is found here.

  • I eluted RNA and DNA samples in 80ul.
  • I’m re-extracting here sample Pacu-H6 that didn’t work on the 9th of December.

Qubit Results

I used Broad range RNA Qubit Protocol inked above. I will check the DNA samples after finishing with all extractions (for all samples). RNA samples were read twice, standard only read once.

  • Standard 1: 445.16
  • Standard 2: 9603.57
Clipping date   Extraction date   colony_id   Species   Temp.Cat   RNA_read1   RNA_read2   RNA_AVG (ng/ul)
20241129   20241210   Pacu-H6   Pocillopora acuta   12   36.6   36.4   36.5
20241203   20241210   Pcom-H9   Porites compressa   30   47.6   46.6   47.1
20241203   20241210   Mcap-G9   Montipora capitata   30   16.8   16.4   16.6
20241129   20241210   Mcap-H6   Montipora capitata   12   22.0   21.6   21.8
  • Standard 1: 451.33
  • Standard 2: 10324.23
Clipping date   Extraction date   colony_id   Species   Temp.Cat   RNA_read1   RNA_read2   RNA_AVG (ng/ul)
20241202   20241210   Mcap-E10   Montipora capitata   26.8   21.2   20.6   20.9
20241129   20241210   Pcom-F6   Porites compressa   12   56.4   55.6   56.0
20241203   20241210   Pacu-G7   Pocillopora acuta   35   39.4   38.6   39.0
20241202   20241210   Pcom-H1   Porites compressa   18   17.4   17.2   17.3

Gel

  • I used the protocol linked above with few modifications. I ran 11ul of RNA and 9ul of DNA. I added 4ul of gel green to the gel.

Gel_DNA_RNA_10Dec2024.png

Written on December 10, 2024