Continuing extracting RNA and DNA from Pacu, Mcap, Pcomp - Hawai TPC - 121224

Extracting RNA and DNA from fragments of Pocillopora acuta, Montipora capitata, Porites compressa for the Hawaii TPC project

Protocols used

I’m continuing extracting RNA and DNA from frozen fragments of Pacuta, Mcap and Pcomp using the Zymo Quick-DNA/RNA Miniprep Plus Kit and the protocol linked above. The kit protocol is found here.

  • I eluted RNA and DNA samples in 80ul.

Qubit Results

I used Broad range RNA Qubit Protocol inked above. I will check the DNA samples after finishing with all extractions (for all samples). RNA samples were read twice, standard only read once.

These 4 extracted on the 11th

  • Standard 1: 442.30
  • Standard 2: 9977.41
Clipping date   Extraction date   colony_id   Species   Temp.Cat   RNA_read1   RNA_read2   RNA_AVG (ng/ul)
20241129   20241211   Pcom-G6   Porites compressa   12   35.8   35.8   35.8
20241203   20241211   Pcom-E9   Porites compressa   30   45.8   46.0   45.9
20241203   20241211   Pacu-F9   Pocillopora acuta   30   34.8   33.4   34.1
20241202   20241211   Mcap-F1   Montipora capitata   18   17.0   17.6   17.3

These 4 extracted on the 12th

  • Standard 1: 459.58
  • Standard 2: 11825.83
Clipping date   Extraction date   colony_id   Species   Temp.Cat   RNA_read1   RNA_read2   RNA_AVG (ng/ul)
20241203   20241212   Pcom-F7   Porites compressa   35   43.4   43.4   43.4
20241203   20241212   Mcap-E7   Montipora capitata   35   57.6   56.8   57.2
20241203   20241212   Mcap-F9   Montipora capitata   30   17.4   16.4   16.9
20241202   20241212   Pacu-F11   Pocillopora acuta   26.8   19.0   19.0   19.0

Gel

  • I used the protocol linked above with few modifications. I ran 11ul of RNA and 9ul of DNA. I added 4ul of gel green to the gel.

Gel_DNA_RNA_12Dec2024.png

Written on December 12, 2024