Sample prep for physiology - Bermuda TPC samples
Preparing Coral Homogenate for Physiological Analyses for the Bermuda TPC ENCORE project
Protocols used
Coral samples of the species Diploria labyrinthiformis (Dlab), Montastraea cavernosa (Mcav), Madracis decactis (Mdec), and Porites astreoides (Past) were airbrushed mainly by Joel Hartery and Pooja Pednekar.
Tissue homogenate was divided into aliquots as follows:
- Chlorophyll - 1.5 ml
- Symbiont Density - 1.5 ml
- Protein - 1.5 ml
- Lipids - 1.5 ml
- Carbohydrates - 1.5 ml
- Ashe-free dry weight - 5 ml
- Spare - 1 ml at least (if enough tissue)
Sample list
| Date | Sample name | Temp Cat | Volum of PBS added (ml) | ||||||
| 20250715 | Past-C3 | 33 | 45 | ||||||
| 20250716 | Dlab-A3 | 33 | 35 | ||||||
| 20250716 | Dlab-D3 | 33 | 34 | ||||||
| 20250721 | Mcav-D3 | 33 | 41 | ||||||
| 20250723 | Mdec-E3 | 33 | 42.5 | ||||||
| 20250723 | Dlab-B3 | 33 | 22.5 | ||||||
| 20250723 | Mcav-C7 | 36 | 38 | ||||||
| 20250730 | Dlab-E7 | 36 | 35 | ||||||
| 20250730 | Past-A7 | 36 | 25 | ||||||
| 20250730 | Mdec-D7 | 36 | 15 | ||||||
| 20250730 | Past-B7 | 36 | 27.5 | ||||||
| 20250730 | Dlab-C7 | 36 | 25 | ||||||
| 20250731 | Mdec-D6 | 26 | 26 | ||||||
| 20250731 | Dlab-F6 | 26 | 23 | ||||||
| 20250731 | Past-E6 | 26 | 16 | ||||||
| 20250731 | Dlab-B6 | 26 | 24 | ||||||
| 20250731 | Mcav-A6 | 26 | 15 | ||||||
| 20250806 | Past-B6 | 26 | 40 | ||||||
| 20250806 | Mcav-E6 | 26 | 27.5 | ||||||
| 20250806 | Mdec-A7 | 36 | 20 | ||||||
| 20250806 | Dlab-D6 | 26 | 20 | ||||||
| 20250806 | Mcav-A7 | 36 | 20 | ||||||
| 20250806 | Mdec-C4 | 16 | 20 | ||||||
| 20250806 | Past-D4 | 16 | 25 | ||||||
| 20250806 | Mcav-A4 | 16 | 17.5 | ||||||
| 20250807 | Mdec-F4 | 16 | 25 | ||||||
| 20250807 | Past-B4 | 16 | 19 | ||||||
| 20250807 | Dlab-A6 | 26 | 20 | ||||||
| 20250807 | Mcav-B6 | 26 | 25 | ||||||
| 20250807 | Mcav-B4 | 16 | 17.5 | ||||||
| 20250807 | Dlab-C4 | 16 | 15 | ||||||
| 20250807 | Dlab-E4 | 16 | 25 | ||||||
| 20250808 | Mdec-C6 | 26 | 25 | ||||||
| 20250808 | Mcav-F6 | 26 | 19 | ||||||
| 20250808 | Past-D6 | 26 | 20 | ||||||
| 20250808 | Past-F7 | 36 | 25 | ||||||
| 20250808 | Dlab-F7 | 36 | 22.5 | ||||||
| 20250808 | Past-A6 | 26 | 15 | ||||||
| 20250808 | Dlab-A7 | 36 | 22.5 | ||||||
| 20250808 | Past-C7 | 36 | 17.5 | ||||||
| 20250808 | Mcav-B7 | 36 | 17.5 | ||||||
| 20250808 | Mdec-E7 | 36 | 15 | ||||||
| 20250808 | Mdec-F6 | 26 | 15 | ||||||
| 20250808 | Mdec-C7 | 36 | 17.5 | ||||||
| 20250808 | Dlab-D7 | 36 | 14 | ||||||
| 20250812 | Mcav-D7 | 36 | 20 | ||||||
| 20250812 | Mdec-B7 | 36 | 23 | ||||||
| 20250812 | Past-D7 | 36 | 17.5 | ||||||
| 20250812 | Past-E7 | 36 | 17.5 | ||||||
| 20250812 | Dlab-B7 | 36 | 20 | ||||||
| 20250812 | Mcav-F7 | 36 | 20 | ||||||
| 20250812 | Mdec-F7 | 36 | 20 | ||||||
| 20250813 | Mcav-C6 | 26 | 25 | ||||||
| 20250813 | Dlab-C6 | 26 | 17.5 | ||||||
| 20250813 | Mcav-D6 | 26 | 20 | ||||||
| 20250813 | Dlab-E6 | 26 | 22 | ||||||
| 20250813 | Past-C6 | 26 | 17.5 | ||||||
| 20250813 | Past-F6 | 26 | 17.5 | ||||||
| 20250813 | Mdec-B6 | 26 | 40 | ||||||
| 20250813 | Mdec-E6 | 26 | 17.5 | ||||||
| 20250814 | Mdec-F3 | 33 | 40 | ||||||
| 20250814 | Past-F3 | 33 | 15 | ||||||
| 20250814 | Mcav-E3 | 33 | 50 | ||||||
| 20250814 | Dlab-E3 | 33 | 36 | ||||||
| 20250814 | Mcav-C3 | 33 | 34.5 | ||||||
| 20250814 | Mdec-D3 | 33 | 52 | ||||||
| 20250814 | Past-A3 | 33 | 35 | ||||||
| 20250814 | Past-B3 | 33 | 40 | ||||||
| 20250814 | Dlab-D4 | 16 | 37.5 | ||||||
| 20250814 | Mdec-A3 | 33 | 47.5 | ||||||
| 20250826 | Dlab-A4 | 16 | 25 | ||||||
| 20250826 | Mcav-E4 | 16 | 27.5 | ||||||
| 20250826 | Mdec-A4 | 16 | 23 | ||||||
| 20250826 | Past-C4 | 16 | 28 | ||||||
| 20250826 | Mdec-B4 | 16 | 10 | ||||||
| 20250826 | Mcav-D4 | 16 | 23.5 | ||||||
| 20250826 | Past-A4 | 16 | 23 | ||||||
| 20250904 | Mcav-F4 | 16 | 19 | ||||||
| 20250904 | Dlab-F4 | 16 | 29 | ||||||
| 20250904 | Mdec-D4 | 16 | 29 | ||||||
| 20250904 | Past-E4 | 16 | No Homogenate | ||||||
| 20250904 | Past-F4 | 16 | 28 | ||||||
| 20250918 | Mcav-A5 | 22 | 32.5 | ||||||
| 20250918 | Dlab-A5 | 22 | 32 | ||||||
| 20250918 | Dlab-B4 | 16 | 31 | ||||||
| 20250918 | Mcav-C4 | 16 | 36 | ||||||
| 20250918 | Past-E5 | 22 | 22.5 | ||||||
| 20250919 | Mcav-F3 | 33 | 27.5 | ||||||
| 20250919 | Mcav-A3 | 33 | 25.5 | ||||||
| 20250919 | Dlab-C3 | 33 | 25 | ||||||
| 20250919 | Dlab-F3 | 33 | 21 | ||||||
| 20250925 | Mdec-D3 | 33 | 43 | ||||||
| 20250925 | Mdec-C3 | 33 | 15 | ||||||
| 20250925 | Past-D3 | 33 | 22 | ||||||
| 20250925 | Past-E3 | 33 | 10.5(nospare)(< 5 ml for dry wt) | ||||||
| 20251003 | Mdec-A1 | 29 | 24 | ||||||
| 20251003 | Mcav-B1 | 29 | 27.5 | ||||||
| 20251003 | Past-A1 | 29 | 34 | ||||||
| 20251003 | Mdec-B1 | 29 | 21 | ||||||
| 20251003 | Dlab-B1 | 29 | 15 | ||||||
| 20251017 | Mcav-A1 | 29 | 37.5 | ||||||
| 20251017 | Dlab-A1 | 29 | 21 | ||||||
| 20251017 | Mdec-F1 | 29 | 30 | ||||||
| 20251017 | Dlab-F1 | 29 | 45 | ||||||
| 20251017 | Mdec-E1 | 29 | 27.5 | ||||||
| 20251020 | Mcav-C1 | 29 | 42 | ||||||
| 20251020 | Dlab-D1 | 29 | 28 | ||||||
| 20251020 | Mdec-C1 | 29 | 47 | ||||||
| 20251020 | Dlab-E1 | 29 | 22 | ||||||
| 20251020 | Past-C1 | 29 | 30 | ||||||
| 20251020 | Mdec-D1 | 29 | 18 | ||||||
| 20251020 | Past-F1 | 29 | 32.5 | ||||||
| 20251020 | Past-E1 | 29 | 35 | ||||||
| 20251020 | Mcav-E1 | 29 | 33 | ||||||
| 20251020 | Mcav-D1 | 29 | 20 | ||||||
| 20251020 | Past-D1 | 29 | 20 | ||||||
| 20251020 | Mcav-F1 | 29 | 27.5 | ||||||
| 20251022 | Mdec-C2 | 40 | 15 | ||||||
| 20251022 | Mcav-D2 | 40 | 15 | ||||||
| 20251022 | Mdec-E2 | 40 | 7 | Only aliquots for chl, pro, carb, ~2ml Spare | |||||
| 20251022 | Past-F2 | 40 | 20 | ||||||
| 20251022 | Dlab-D2 | 40 | 19 | ||||||
| 20251022 | Mcav-E2 | 40 | 22 | ||||||
| 20251029 | Mcav-A2 | 40 | 15 | ||||||
| 20251029 | Mcav-B2 | 40 | 20 | ||||||
| 20251029 | Past-C2 | 40 | 15 | ||||||
| 20251029 | Past-B2 | 40 | 15 | ||||||
| 20251029 | Dlab-F2 | 40 | 17.5 | ||||||
| 20251029 | Past-A2 | 40 | 16.0 | ||||||
| 20251029 | Dlab-A2 | 40 | 21.0 | ||||||
| 20251029 | Dlab-B2 | 40 | 16.0 | ||||||
| 20251030 | Mcav-E5 | 22 | 17.5 | ||||||
| 20251030 | MCAV-F5 | 22 | 17.5 | ||||||
| 20251030 | PAST-D5 | 22 | 14.0 | ||||||
| 20251030 | PAST-B5 | 22 | 15.0 | ||||||
| 20251030 | MCAV-B5 | 22 | 22.5 | ||||||
| 20251030 | PAST-A5 | 22 | 15.0 | ||||||
| 20251030 | DLAB-B5 | 22 | 16.0 | ||||||
| 20251030 | DLAB-D5 | 22 | 15.0 | ||||||
| 20251030 | MDEC-E5 | 22 | 21.0 | ||||||
| 20251030 | DLAB-C5 | 22 | 20.0 | ||||||
| 20251030 | MDEC-A5 | 22 | 9.0 | None left for dry weight and spare | |||||
| 20251030 | MDEC-B5 | 22 | 22.5 | ||||||
| 20251030 | DLAB-E2 | 40 | 17.5 | ||||||
| 20251030 | MDEC-F2 | 40 | 9.0 | None left for dry weight and spare | |||||
| 20251030 | DLAB-C2 | 40 | 12.5 | No Spare | |||||
| 20251030 | MDEC-D2 | 40 | 25.0 | ||||||
| 20251031 | PAST-E2 | 40 | 15.0 | ||||||
| 20251031 | PAST-D2 | 40 | 15.0 | ||||||
| 20251031 | PAST-C5 | 22 | 17.5 | ||||||
| 20251031 | MCAV-F2 | 40 | 20.0 | ||||||
| 20251031 | MCAV-C5 | 22 | 20.0 | ||||||
| 20251031 | DLAB-F5 | 22 | 22.5 | ||||||
| 20251031 | MCAV-C2 | 40 | 22.5 | ||||||
| 20251031 | MCAV-D5 | 22 | 12.5 | No spare left | |||||
| 20251031 | DLAB-E5 | 22 | 15.0 | ||||||
| 20251031 | PAST-F5 | 22 | 16.5 | ||||||
| 20251031 | MDEV-D5 | 22 | 17.5 | ||||||
| 20251031 | MDEC-F5 | 22 | 17.5 |
Written on September 11, 2025