Preparing Coral Homogenate for Physiological Analyses for the Bermuda TPC ENCORE project

Protocols used

• Airbrushing protocol

Coral samples of the species Diploria labyrinthiformis (Dlab), Montastraea cavernosa (Mcav), Madracis decactis (Mdec), and Porites astreoides (Past) were airbrushed mainly by Joel Hartery and Pooja Pednekar.

Tissue homogenate was divided into aliquots as follows:

• Chlorophyll - 1.5 ml
• Symbiont Density - 1.5 ml
• Protein - 1.5 ml
• Lipids - 1.5 ml
• Carbohydrates - 1.5 ml
• Ashe-free dry weight - 5 ml
• Spare - 1 ml at least (if enough tissue)

Sample list